Difference between revisions of "Part:BBa K2938000"
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[[File:Larva GFP.png|200px|thumb|left|Mosquitoes' larva that was fed with serratia marcescens 274 expressing plasmid with Cry11Aa and deGFP.]] | [[File:Larva GFP.png|200px|thumb|left|Mosquitoes' larva that was fed with serratia marcescens 274 expressing plasmid with Cry11Aa and deGFP.]] | ||
| + | [[File:Cry11Aa_Western.png|200px|thumb|right|Western Blot of Bacteria expressing Cry11Aa and deGFP – proof of Cry11Aa expression]] | ||
Revision as of 18:03, 10 October 2019
Long Description
PR1 promoter is an attenuated promoter of the pBEST plasmid’s PR promoter. It can be used in a polycistronic plasmid to express more than one gene. It can be used to get lower expression levels of genes than the original PR promoter, which can sometimes help the protein expression and was shown to be more effective during in-vivo gene expression. Our devices contain this promoter, alongside Bti toxin subunits and deGFP, thus expressing toxins with functional usage and deGFP for tracking of our bacteria.
Usage and Biology
Promoters are regulatory DNA parts, which can be used to express genes.
Characterization
The PR1 promoter can be expressed in E. Coli and Serratia Marcescens. In the following photos, we see genes expressed from the polycistronic plasmid, which our PR1 promoter is it’s promoter in Serratia Marcescens 274 fed to Aedes Aegypti mosquitoes larvae.
