Difference between revisions of "Part:BBa K2934000"

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[[File:Glucose_Oxidase_Reaction.png|500px|thumb|center|figure 1: the biochemical reaction of glucose oxidase]]
 
[[File:Glucose_Oxidase_Reaction.png|500px|thumb|center|figure 1: the biochemical reaction of glucose oxidase]]
  
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Glucose oxidase catalyzes the oxidation of glucose to using molecular oxygen to create hydrogen peroxide and D-glucono lactone. It requires the cofactor, flavin adenine dinucleotide (FAD). FAD acts as the initial electron acceptor, in a redox reaction, and is reduced to FADH2. Then FADH2 is oxidized by molecular oxygen, which is then reduced to hydrogen peroxide [1].
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Glucose oxidase (GOx) catalyzes the oxidation of glucose to using molecular oxygen to create hydrogen peroxide and D-glucono lactone. GOx requires the cofactor flavin adenine dinucleotide (FAD) that acts as an electron acceptor in a redox reaction. It is reduced to FADH2, then it is oxidized by molecular oxygen, which is then reduced to hydrogen peroxide [1].
  
 
===References===
 
===References===

Revision as of 15:25, 10 October 2019


Glucose Oxidase-Histag A. niger optimized for B. subtilis

Glucose Oxidase gene is coding for the Glucose Oxidase (GOx) enzyme, which catalyzes the oxidation of glucose to D-glucono-lacton and release of hydrogen peroxide. The enzyme origin is from A. niger and is optimized for B. subtilis.

Usage and Biology

figure 1: the biochemical reaction of glucose oxidase

Glucose oxidase (GOx) catalyzes the oxidation of glucose to using molecular oxygen to create hydrogen peroxide and D-glucono lactone. GOx requires the cofactor flavin adenine dinucleotide (FAD) that acts as an electron acceptor in a redox reaction. It is reduced to FADH2, then it is oxidized by molecular oxygen, which is then reduced to hydrogen peroxide [1].

References

[1] Frederick KR, Tung J, Richard S. Emerick F, Chamberlain SH, Vasavada A, Steven R, Chakraborty S, Schopter LM, Schopter LM, Massey V. 1990. Glucose Oxidase from Aspergillus niger. J Biol Chem 265:3793–3802.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 30
    Illegal BsaI site found at 361
    Illegal BsaI.rc site found at 307
    Illegal BsaI.rc site found at 1132