Difference between revisions of "Part:BBa K2934000"

Revision as of 15:12, 10 October 2019

Glucose Oxidase-Histag A. niger optimized for B. subtilis

Glucose Oxidase gene is coding for the Glucose Oxidase (GOx) enzyme, which catalyzes the oxidation of glucose to D-glucono-lacton and release of hydrogen peroxide. The enzyme origin is from A. niger and is optimized for B. subtilis.

Usage and Biology

alt text

Glucose oxidase catalyzes the oxidation of glucose to using molecular oxygen to create hydrogen peroxide and D-glucono lactone. It requires the cofactor, flavin adenine dinucleotide (FAD). FAD acts as the initial electron acceptor, in a redox reaction, and is reduced to FADH2. Then FADH2 is oxidized by molecular oxygen, which is then reduced to hydrogen peroxide [1].

References

[1] Frederick KR, Tung J, Richard S. Emerick F, Chamberlain SH, Vasavada A, Steven R, Chakraborty S, Schopter LM, Schopter LM, Massey V. 1990. Glucose Oxidase from Aspergillus niger. J Biol Chem 265:3793–3802.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 30
Illegal BsaI site found at 361
Illegal BsaI.rc site found at 307
Illegal BsaI.rc site found at 1132

@@ Line 8: / Line 8: @@
 [[File:Glucose_Oxidase_Reaction.png|500px|thumb|center|alt text]]
-Glucose oxidase catalyzes the oxidation of glucose to using molecular oxygen to create hydrogen peroxide and D-glucono lactone.
+Glucose oxidase catalyzes the oxidation of glucose to using molecular oxygen to create hydrogen peroxide and D-glucono lactone.  It requires the cofactor, flavin adenine dinucleotide (FAD). FAD acts as the initial electron acceptor, in a redox reaction, and is reduced to FADH2. Then FADH2 is oxidized by molecular oxygen, which is then reduced to hydrogen peroxide [1].
+===References===
+[1] Frederick KR, Tung J, Richard S. Emerick F, Chamberlain SH, Vasavada A, Steven R, Chakraborty S, Schopter LM, Schopter LM, Massey V. 1990. Glucose Oxidase from Aspergillus niger. J Biol Chem 265:3793–3802.
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