Difference between revisions of "Part:BBa K2934004:Design"
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The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (<em>B. subtilis</em> Secretory Protein Expression System) [1]. | The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (<em>B. subtilis</em> Secretory Protein Expression System) [1]. | ||
===References=== | ===References=== | ||
[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system | [1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system | ||
Revision as of 14:32, 10 October 2019
AmyE - Glucose Oxidase-Histag for Bacillus subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 156
Illegal BsaI site found at 487
Illegal BsaI.rc site found at 433
Illegal BsaI.rc site found at 1258
Design Notes
PstI restriction site at 1947, XbaI restriction site at 1953.
Source
AmyE - BBa_K2273023
GOx - BBa_K2934000
Linker - BBa_K2934005
The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (B. subtilis Secretory Protein Expression System) [1].