Difference between revisions of "Part:BBa K2934004:Design"

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AmyE - BBa_K2273023
 
AmyE - BBa_K2273023
 
GOx - BBa_K2934000
 
GOx - BBa_K2934000
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The amyE signal peptide is connected to the GOx enzyme with a nine amino acids linker, and the GOx enzyme is connected to the Histag with a seven amino acids linker. Both linkers and Histag are based on the pBE-S commercial plasmid made by Takara (https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system).  
+
Linker - BBa_K2934005
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The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (<em>B. subtilis</em> Secretory Protein Expression System) [1].
  
 
===References===
 
===References===
 
[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system
 
[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system

Revision as of 14:32, 10 October 2019


AmyE - Glucose Oxidase-Histag for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 487
    Illegal BsaI.rc site found at 433
    Illegal BsaI.rc site found at 1258


Design Notes

PstI restriction site at 1947, XbaI restriction site at 1953.


Source

AmyE - BBa_K2273023 GOx - BBa_K2934000 Linker - BBa_K2934005 The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (B. subtilis Secretory Protein Expression System) [1].

References

[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system