Difference between revisions of "Part:BBa K2934005:Design"
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===Source=== | ===Source=== | ||
| − | + | The part is taken from the pBE-S commercial plasmid made by Takara (<em>B. subtilis</em> Secretory | |
| − | The part is taken from the pBE-S commercial plasmid made by Takara [1]. | + | Protein Expression System) [1]. |
===References=== | ===References=== | ||
[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system | [1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system | ||
Revision as of 14:30, 10 October 2019
Signal peptide-protein linker for Bacillus subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence starts with an EagI (Eco52I) restriction site, for simple substitution od signal peptide. It contains SacI, Eco53kI and NdeI restriction sites.
Source
The part is taken from the pBE-S commercial plasmid made by Takara (B. subtilis Secretory Protein Expression System) [1].