Difference between revisions of "Part:BBa K2967012:Design"
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===Source=== | ===Source=== | ||
| − | The natural gene regulatory architecture in the hrp control network contains the ingredients of a tunable transcriptional amplifier: The activator proteins HrpR and HrpS form a ultrasensitive high-order co-complex, which | + | The natural gene regulatory architecture in the hrp control network contains the ingredients of a tunable transcriptional amplifier: The activator proteins HrpR and HrpS form a ultrasensitive high-order co-complex, which binds the upstream activator sequence of the hrpL promoter to remodel the closed σ54 -RNAP-hrpL transcription complex to an open one through ATP hydrolysis.[2] |
===References=== | ===References=== | ||
[2] Levskaya,A., Weiner,O.D., Lim,W.A. and Voigt,C.A. (2009) Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature, 461, 997–1001. | [2] Levskaya,A., Weiner,O.D., Lim,W.A. and Voigt,C.A. (2009) Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature, 461, 997–1001. | ||
Latest revision as of 10:41, 10 October 2019
The fixed-gain bio-amplifier
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2102
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1312
Illegal BsaI.rc site found at 3047
Illegal SapI.rc site found at 1945
Design Notes
In order to quickly test the effect of our amplifier, we used T7 promoter with strong transcriptional activity.
Source
The natural gene regulatory architecture in the hrp control network contains the ingredients of a tunable transcriptional amplifier: The activator proteins HrpR and HrpS form a ultrasensitive high-order co-complex, which binds the upstream activator sequence of the hrpL promoter to remodel the closed σ54 -RNAP-hrpL transcription complex to an open one through ATP hydrolysis.[2]
References
[2] Levskaya,A., Weiner,O.D., Lim,W.A. and Voigt,C.A. (2009) Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature, 461, 997–1001.