Difference between revisions of "Part:BBa K2967011"

Line 4: Line 4:
  
 
It is the sequence of inducible promoter hrpL. HrpR(BBa_K2967008) protein binds to HrpS(BBa_K2967009) protein forming a complex and then triggering the transcription of induced promoter hrpL. It functions like a AND logic gate to open an gene circuit.
 
It is the sequence of inducible promoter hrpL. HrpR(BBa_K2967008) protein binds to HrpS(BBa_K2967009) protein forming a complex and then triggering the transcription of induced promoter hrpL. It functions like a AND logic gate to open an gene circuit.
the hrpL promoter (PhrpL) contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays.  
+
The hrpL promoter (PhrpL) contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:28, 10 October 2019


The inducible promoter, PhrpL

It is the sequence of inducible promoter hrpL. HrpR(BBa_K2967008) protein binds to HrpS(BBa_K2967009) protein forming a complex and then triggering the transcription of induced promoter hrpL. It functions like a AND logic gate to open an gene circuit. The hrpL promoter (PhrpL) contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]