Difference between revisions of "Part:BBa K3254018"
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<partinfo>BBa_K3254018 short</partinfo> | <partinfo>BBa_K3254018 short</partinfo> | ||
| − | This sequence was | + | This is a conservative design of phiC31 integrase coding sequence. This sequence was isolated from a streptomyces shuttle vector and the EcoRI site was removed. Comparing to [[Part:BBa_K1039012|BBa_K1039012]], this design has a longer coding region, and the "atg" start codon was replaced by "gtg" codon. Because there is an start codon in the upstream, some researchers likely annotated it as a part of the coding sequence. Here we design this part and placed it after a RiboJ([[Part:BBa_K1679038|BBa_K1679038]]) sequence for achieving a appropriate response interval of phiC31 integrase. |
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Revision as of 10:14, 10 October 2019
original sequence of phiC31 integrase gene
This is a conservative design of phiC31 integrase coding sequence. This sequence was isolated from a streptomyces shuttle vector and the EcoRI site was removed. Comparing to BBa_K1039012, this design has a longer coding region, and the "atg" start codon was replaced by "gtg" codon. Because there is an start codon in the upstream, some researchers likely annotated it as a part of the coding sequence. Here we design this part and placed it after a RiboJ(BBa_K1679038) sequence for achieving a appropriate response interval of phiC31 integrase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 75
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1689
Illegal SapI.rc site found at 1380
Illegal SapI.rc site found at 1438
Illegal SapI.rc site found at 1543