Difference between revisions of "Part:BBa K3038000:Experience"

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[[Image:T--Poitiers--PCR amplification ADR-tab3.jpg|400px|thumb|center| Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3.
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https://2019.igem.org/File:T--Poitiers--PCR_amplification_ADR-tab3.jpg
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Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3.
 
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Plasmid construction
 
Plasmid construction
  
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https://2019.igem.org/wiki/images/5/5b/T--Poitiers--plasmid_construction_ADR-tab3.jpg
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https://2019.igem.org/File:T--Poitiers--plasmid_construction_ADR-tab3.jpg
  
 
Design of ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.  
 
Design of ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.  

Revision as of 09:00, 10 October 2019


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Please enter how you used this part and how it worked out.

Applications of BBa_K3038000

PCR amplification of the PCR products


https://2019.igem.org/File:T--Poitiers--PCR_amplification_ADR-tab3.jpg


Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3. ]]


Plasmid construction

https://2019.igem.org/File:T--Poitiers--plasmid_construction_ADR-tab3.jpg

Design of ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.



Expression of the recombinant protein


Activity

User Reviews

UNIQf19e80bec6807c94-partinfo-00000000-QINU UNIQf19e80bec6807c94-partinfo-00000001-QINU