Difference between revisions of "Part:BBa K2938018"
Line 3: Line 3:
 
<partinfo>BBa_K2938018 short</partinfo>
 
<partinfo>BBa_K2938018 short</partinfo>
  
−
PR1 - RBS - Cry4Ba (HA tag) - RBS - Cry11Aa (Strep tag) - RBS - Cyt1Aa (Strep tag) - RBS - P20 (His tag) - RBS -  deGFP (His tag) - T500 Terminator.  
+
PR1 - RBS - Cry4Ba (HA tag) - RBS - Cry11Aa (Strep tag) - RBS - Cyt1Aa (Strep tag) - RBS - P20 (His tag) - RBS -  deGFP - T500 Terminator.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:27, 9 October 2019


Polycistronic BTI Toxin

PR1 - RBS - Cry4Ba (HA tag) - RBS - Cry11Aa (Strep tag) - RBS - Cyt1Aa (Strep tag) - RBS - P20 (His tag) - RBS - deGFP - T500 Terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1846
    Illegal EcoRI site found at 2142
    Illegal EcoRI site found at 6316
    Illegal EcoRI site found at 6627
    Illegal XbaI site found at 3820
    Illegal XbaI site found at 5510
    Illegal SpeI site found at 958
    Illegal SpeI site found at 4509
    Illegal PstI site found at 2324
    Illegal PstI site found at 5976
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1846
    Illegal EcoRI site found at 2142
    Illegal EcoRI site found at 6316
    Illegal EcoRI site found at 6627
    Illegal NheI site found at 56
    Illegal NheI site found at 105
    Illegal NheI site found at 2093
    Illegal NheI site found at 2208
    Illegal SpeI site found at 958
    Illegal SpeI site found at 4509
    Illegal PstI site found at 2324
    Illegal PstI site found at 5976
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1846
    Illegal EcoRI site found at 2142
    Illegal EcoRI site found at 6316
    Illegal EcoRI site found at 6627
    Illegal BglII site found at 6274
    Illegal BamHI site found at 5653
    Illegal XhoI site found at 7655
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1846
    Illegal EcoRI site found at 2142
    Illegal EcoRI site found at 6316
    Illegal EcoRI site found at 6627
    Illegal XbaI site found at 3820
    Illegal XbaI site found at 5510
    Illegal SpeI site found at 958
    Illegal SpeI site found at 4509
    Illegal PstI site found at 2324
    Illegal PstI site found at 5976
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1846
    Illegal EcoRI site found at 2142
    Illegal EcoRI site found at 6316
    Illegal EcoRI site found at 6627
    Illegal XbaI site found at 3820
    Illegal XbaI site found at 5510
    Illegal SpeI site found at 958
    Illegal SpeI site found at 4509
    Illegal PstI site found at 2324
    Illegal PstI site found at 5976
    Illegal NgoMIV site found at 4632
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2614