Difference between revisions of "Part:BBa K3102008:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
We mutated a Thymine into Cysteine at the 844bp position (T844C) because of the PstI enzyme
 
We mutated a Thymine into Cysteine at the 844bp position (T844C) because of the PstI enzyme
−
 
−
PstI
 
−
the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct.
 
  
 
===Source===
 
===Source===

Revision as of 16:16, 9 October 2019


ZWF (Glucose-6-phosphate 1-dehydrogenase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 775
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 775
    Illegal NotI site found at 656
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 775
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 775
    Illegal NgoMIV site found at 48
    Illegal NgoMIV site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated a Thymine into Cysteine at the 844bp position (T844C) because of the PstI enzyme

Source

Corynebacterium glutamicum, sequence found through Ebi https://www.ebi.ac.uk/ena/data/view/BAB98969

References

"Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate." Zhao J., Baba T., Mori H., Shimizu K. Metab. Eng. 6:164-174(2004)