Difference between revisions of "Part:BBa K3102008:Design"

Revision as of 16:16, 9 October 2019

ZWF (Glucose-6-phosphate 1-dehydrogenase)

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 775
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 775
Illegal NotI site found at 656
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1332
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 775
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 775
Illegal NgoMIV site found at 48
Illegal NgoMIV site found at 765
1000
COMPATIBLE WITH RFC[1000]

Design Notes

We mutated a Thymine into Cysteine at the 844bp position (T844C) because of the PstI enzyme

PstI the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct.

Source

Corynebacterium glutamicum, sequence found through Ebi https://www.ebi.ac.uk/ena/data/view/BAB98969

References

"Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate." Zhao J., Baba T., Mori H., Shimizu K. Metab. Eng. 6:164-174(2004)

@@ Line 7: / Line 7: @@
 ===Design Notes===
-We mutated a Thymine into Cysteine at the 844bp position (T844C) because of XXXXXXXXXXXXX
+We mutated a Thymine into Cysteine at the 844bp position (T844C) because of the PstI enzyme
 PstI