Difference between revisions of "Part:BBa K3102008:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 9: Line 9:
 
We mutated a Thymine into Cysteine at the 844bp position (T844C) because of XXXXXXXXXXXXX
 
We mutated a Thymine into Cysteine at the 844bp position (T844C) because of XXXXXXXXXXXXX
  
−
 
+
PstI
 
the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct.
 
the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct.
  

Revision as of 14:52, 9 October 2019


ZWF (Glucose-6-phosphate 1-dehydrogenase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 775
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 775
    Illegal NotI site found at 656
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 775
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 775
    Illegal NgoMIV site found at 48
    Illegal NgoMIV site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated a Thymine into Cysteine at the 844bp position (T844C) because of XXXXXXXXXXXXX

PstI the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct.

Source

Corynebacterium glutamicum, sequence found through Ebi https://www.ebi.ac.uk/ena/data/view/BAB98969

References

"Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate." Zhao J., Baba T., Mori H., Shimizu K. Metab. Eng. 6:164-174(2004)