Difference between revisions of "Part:BBa K2205001"
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Through integrating engineered Serratia containing a polycistronic plasmid with deGFP, we can track the spread of our engineered bacteria and assume the expression of the other genes from the polycistronic plasmid are occurring as well. | Through integrating engineered Serratia containing a polycistronic plasmid with deGFP, we can track the spread of our engineered bacteria and assume the expression of the other genes from the polycistronic plasmid are occurring as well. | ||
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Revision as of 14:37, 9 October 2019
deGFP
deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants.
Characterisation data for deGFP for collected using the expression construct BBa_K2205002
CFPS Expression of deGFP
CFPS Expression of J23100-deGFP and J23100-sfGFP Constructs: Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.
In vivo Qualitative Comparison
FACS Data - Collected by Exeter iGEM 2017 for Newcastle iGEM 2017
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Figure 5: Single cell data for E. coli cells expressing deGFP (collected by Exeter iGEM). Columns 1-6: cells expressing deGFP. Columns 7 and 8: wild type Top10 cells. Red parts of the bar are non-fluorescent cells, green parts are fluorescent cells.
Characterization by 2019 BGU_Israel team:
The deGFP can be expressed in a polycistronic plasmid and is expressed and translated in Serratia Marcescens. The deGFP can be used as a reporter gene in mosquito larvae, as they are transparent, and the Serratia are part of their microbiome.
Through integrating engineered Serratia containing a polycistronic plasmid with deGFP, we can track the spread of our engineered bacteria and assume the expression of the other genes from the polycistronic plasmid are occurring as well.
