Difference between revisions of "Part:BBa K2205001"
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<img src="https://static.igem.org/mediawiki/2017/c/cc/T--Newcastle--BB_deGFP_FACS.png" width="600px"/> | <img src="https://static.igem.org/mediawiki/2017/c/cc/T--Newcastle--BB_deGFP_FACS.png" width="600px"/> | ||
Figure 5: Single cell data for '''E. coli''' cells expressing deGFP (collected by Exeter iGEM). Columns 1-6: cells expressing deGFP. Columns 7 and 8: wild type Top10 cells. Red parts of the bar are non-fluorescent cells, green parts are fluorescent cells. | Figure 5: Single cell data for '''E. coli''' cells expressing deGFP (collected by Exeter iGEM). Columns 1-6: cells expressing deGFP. Columns 7 and 8: wild type Top10 cells. Red parts of the bar are non-fluorescent cells, green parts are fluorescent cells. | ||
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| + | ===Characterization by 2019 BGU_Israel team: === | ||
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| + | The deGFP can be expressed in a polycistronic plasmid and is expressed and translated in Serratia Marcescens. The deGFP can be used as a reporter gene in mosquito larvae, as they are transparent, and the Serratia are part of their microbiome. | ||
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| + | Through integrating engineered Serratia containing a polycistronic plasmid with deGFP, we can track the spread of our engineered bacteria and assume the expression of the other genes from the polycistronic plasmid are occurring as well. | ||
Revision as of 14:35, 9 October 2019
deGFP
deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants.
Characterisation data for deGFP for collected using the expression construct BBa_K2205002
CFPS Expression of deGFP
CFPS Expression of J23100-deGFP and J23100-sfGFP Constructs: Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.
In vivo Qualitative Comparison
FACS Data - Collected by Exeter iGEM 2017 for Newcastle iGEM 2017
<img src="
" width="600px"/>
Figure 5: Single cell data for E. coli cells expressing deGFP (collected by Exeter iGEM). Columns 1-6: cells expressing deGFP. Columns 7 and 8: wild type Top10 cells. Red parts of the bar are non-fluorescent cells, green parts are fluorescent cells.
Characterization by 2019 BGU_Israel team:
The deGFP can be expressed in a polycistronic plasmid and is expressed and translated in Serratia Marcescens. The deGFP can be used as a reporter gene in mosquito larvae, as they are transparent, and the Serratia are part of their microbiome.
Through integrating engineered Serratia containing a polycistronic plasmid with deGFP, we can track the spread of our engineered bacteria and assume the expression of the other genes from the polycistronic plasmid are occurring as well.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]