Difference between revisions of "Part:BBa K3254001:Design"

 
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===References===
 
===References===
 +
Chen, Y.J., et al., Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods, 2013. 10(7): p. 659-64.
 +
Yang, L., et al., Permanent genetic memory with >1-byte capacity. Nat Methods, 2014. 11(12): p. 1261-6.

Latest revision as of 09:35, 9 October 2019


Int5attP-BsaI site-terminator-Int5attB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 74
    Illegal BsaI.rc site found at 62


Design Notes

This part sequence was tested in silico before synthesised to avoid potential promoter activity (see the website in references). It also should be noted that the sequence of attP and attB sites are in the same deriction.


Source

This part was totally synthesised by commerical company.

References

Chen, Y.J., et al., Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods, 2013. 10(7): p. 659-64. Yang, L., et al., Permanent genetic memory with >1-byte capacity. Nat Methods, 2014. 11(12): p. 1261-6.