Difference between revisions of "Part:BBa K2934000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The sequence is based on the <em>A. niger</em> glucose oxidase (GOx) amino acid sequence and codon optimization for <em>B. subtilis</em>, using codon usage tables | + | The sequence is based on the <em>A. niger</em> glucose oxidase (GOx) amino acid sequence and codon optimization for <em>B. subtilis</em>, using codon usage tables [2] [3]. |
===Source=== | ===Source=== |
Revision as of 12:37, 8 October 2019
Glucose Oxidase-Histag A. niger optimized for B. subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 30
Illegal BsaI site found at 361
Illegal BsaI.rc site found at 307
Illegal BsaI.rc site found at 1132
Design Notes
The sequence is based on the A. niger glucose oxidase (GOx) amino acid sequence and codon optimization for B. subtilis, using codon usage tables [2] [3].
Source
The amino acid sequence was taken from UniProt (https://www.uniprot.org/uniprot/P13006), the gene sequence is based on the amino acid sequence.
References
[1] https://www.uniprot.org/uniprot/P13006 [2] https://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1423 [3] http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=224308&aa=1&style=N