Difference between revisions of "Part:BBa K3185004"
0012massan (Talk | contribs) |
0012massan (Talk | contribs) |
||
| Line 3: | Line 3: | ||
<partinfo>BBa_K3185004 short</partinfo> | <partinfo>BBa_K3185004 short</partinfo> | ||
| + | ==Usage and Biology== | ||
BaCBM2 is a Carbonhydrate-binding Module family 2(CBM2) derived from Bacillus anthracis[1]. | BaCBM2 is a Carbonhydrate-binding Module family 2(CBM2) derived from Bacillus anthracis[1]. | ||
CBMs are protein domains mainly found in carbohydrate-active enzymes. | CBMs are protein domains mainly found in carbohydrate-active enzymes. | ||
| Line 11: | Line 12: | ||
| − | |||
| − | |||
| − | |||
| − | |||
<!-- --> | <!-- --> | ||
Revision as of 10:50, 8 October 2019
SPYCatcher -> BaCBM2
Usage and Biology
BaCBM2 is a Carbonhydrate-binding Module family 2(CBM2) derived from Bacillus anthracis[1]. CBMs are protein domains mainly found in carbohydrate-active enzymes.
As our project involves connecting different proteins, we decided to use the SpyTag/SpyCatcher system and have inserted the SpyCatcher at the N-terminal of the BaCBM2.
We insert this part in pET-11a plasmid between BamHI and NdeI restriction sites.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]