Difference between revisions of "Part:BBa K3185004"

Revision as of 10:46, 8 October 2019

SPYCatcher -> BaCBM2

BaCBM2 is a Carbonhydrate-binding Module family 2(CBM2) derived from Bacillus anthracis[1]. CBMs are protein domains mainly found in carbohydrate-active enzymes.

As our project involves connecting different proteins, we decided to use the SpyTag/SpyCatcher system and have inserted the SpyCatcher at the N-terminal of the BaCBM2.

We insert this part in pET-11a plasmid between BamHI and NdeI restriction sites.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 BaCBM2 is a Carbonhydrate-binding Module family 2(CBM2) derived from Bacillus anthracis[1].
 CBMs are protein domains mainly found in carbohydrate-active enzymes.
-In this part, we express BaCBM2 gene downstream of Spy Catcher gene(りんく).
+As our project involves connecting different proteins, we decided to use the SpyTag/SpyCatcher system and have inserted the SpyCatcher at the N-terminal of the BaCBM2.
-Using this part with any protein linked with Spy Tag, you can anchor this part to your objective protein.
-We insert this part in pET-11a plasmid between BamHI
+We insert this part in pET-11a plasmid between BamHI and NdeI restriction sites.
@@ Line 26: / Line 23: @@
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
+<partinfo>BBa_K3185004 short</partinfo>
 <partinfo>BBa_K3185004 parameters</partinfo>
 <!-- -->