Difference between revisions of "Part:BBa K3102014"
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Dong JM et al., Three overlapping lct genes involved in L-lactate utilization by Escherichia coli., Journal of Bacteriology, 1993 Oct;175(20):6671-8. | Dong JM et al., Three overlapping lct genes involved in L-lactate utilization by Escherichia coli., Journal of Bacteriology, 1993 Oct;175(20):6671-8. | ||
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Nunez MF et al., Transport of L-Lactate, D-Lactate, and glycolate by the LldP and GlcA membrane carriers of Escherichia coli, Biochem Biophys Res Commun. 2002 Jan 18;290(2):824-9. | Nunez MF et al., Transport of L-Lactate, D-Lactate, and glycolate by the LldP and GlcA membrane carriers of Escherichia coli, Biochem Biophys Res Commun. 2002 Jan 18;290(2):824-9. | ||
Revision as of 08:21, 8 October 2019
LldP (L-lactate permease)
E.coli, lldP is an inner membrane permease that functions in the uptake of L-lactate, D-lactate and glycolate [Dong et al., 1993; Nunez et al., 2002]. The lldP gene is located in a lactate-inducible operon with the lldD and lldR genes encoding a lactate dehydrogenase and a regulatory protein, respectively. By overexpressing a plasmid carrying this enzyme, the bacteria is able to uptake the double of these three substrates, L-lactate, D-lactate and glycolate.
Dong JM et al., Three overlapping lct genes involved in L-lactate utilization by Escherichia coli., Journal of Bacteriology, 1993 Oct;175(20):6671-8.
Nunez MF et al., Transport of L-Lactate, D-Lactate, and glycolate by the LldP and GlcA membrane carriers of Escherichia coli, Biochem Biophys Res Commun. 2002 Jan 18;290(2):824-9.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 275
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 411
Illegal AgeI site found at 874
Illegal AgeI site found at 1312
Illegal AgeI site found at 1471
Illegal AgeI site found at 1483 - 1000COMPATIBLE WITH RFC[1000]