Difference between revisions of "Part:BBa K3137014"

Revision as of 06:56, 8 October 2019

responce circuit

The respond gene circuit is composed of an inducible promoter PputA and an anti-phage protein gene of the incubation period (about 5 min), and an inducible promoter PglcF and a toxic protein gene kilP of the outbreak period (about 20 min).

When E. coli is infected by a phage, both report and respond gene circuits are induced to express at the same time. When a phage infects E. coli into the incubation period (about 5 min), the inducible promoter PputA will induce the bacteria to express the resistant protein and the green fluorescent protein gene egfp.

If the resistant protein successfully resists phage infection, the inducible promoter PglcF will not induce the expression of downstream gene. If the resistant protein fails to kill phages, it means that phages will continue to infect bacteria, when it comes to the outbreak period (around 20 min) of phage infection, the inducible promoter PglcF will activate the red fluorescent protein gene mCherry and the expression of downstream toxic protein gene so that the bacteria will display red fluoresce and lyse cells before the assembly of phages.

Sequence and Features