Difference between revisions of "Part:BBa K3102024"

 
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<partinfo>BBa_K3102024 short</partinfo>
 
<partinfo>BBa_K3102024 short</partinfo>
  
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This T7-EG1-TEV-hlyA-ter (secreted EG1) composite is engaged into the cellulose acetate breaking down, by synthesize the EG1 (Endo-1,4-beta-glucanase). The TEV (BBa_K3102012) and HlyA basic parts (BBa_K3102015 or BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV part added a TEV recognition sequence, that will be used to remove the signal peptide, HlyA.
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This constructs is enriched with primer, T7 promoters, RBS and terminator.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:52, 7 October 2019


T7-RBS-EG1-ter (secreted EG1)

This T7-EG1-TEV-hlyA-ter (secreted EG1) composite is engaged into the cellulose acetate breaking down, by synthesize the EG1 (Endo-1,4-beta-glucanase). The TEV (BBa_K3102012) and HlyA basic parts (BBa_K3102015 or BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV part added a TEV recognition sequence, that will be used to remove the signal peptide, HlyA.

This constructs is enriched with primer, T7 promoters, RBS and terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1161
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 104
    Illegal AgeI site found at 362
    Illegal AgeI site found at 452
    Illegal AgeI site found at 1289
  • 1000
    COMPATIBLE WITH RFC[1000]