Difference between revisions of "Part:BBa K3185004"
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CBMs are protein domains mainly found in carbohydrate-active enzymes. | CBMs are protein domains mainly found in carbohydrate-active enzymes. | ||
| − | In this part, we express BaCBM2 gene downstream of | + | In this part, we express BaCBM2 gene downstream of Spy Catcher gene(りんく). |
| + | |||
| + | Using this part with any protein linked with Spy Tag, you can anchor this part to your objective protein. | ||
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| + | We insert this part in pET-11a plasmid between BamHI | ||
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Revision as of 14:01, 7 October 2019
SPYCatcher -> BaCBM2
BaCBM2 is a Carbonhydrate-binding Module family 2(CBM2) derived from Bacillus anthracis[1].
CBMs are protein domains mainly found in carbohydrate-active enzymes.
In this part, we express BaCBM2 gene downstream of Spy Catcher gene(りんく).
Using this part with any protein linked with Spy Tag, you can anchor this part to your objective protein.
We insert this part in pET-11a plasmid between BamHI
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]