Difference between revisions of "Part:BBa K2959007:Design"
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===References=== | ===References=== | ||
| + | 1. Odintsova, T. I., Vassilevski, A. A., Slavokhotova, A. A., Musolyamov, A. K., Finkina, E. I., Khadeeva, N. V., … Egorov, T. A. (2009). A novel antifungal hevein-type peptide fromTriticum kiharaeseeds with a unique 10-cysteine motif. FEBS Journal, 276(15), 4266–4275. doi: 10.1111/j.1742-4658.2009.07135.x | ||
Latest revision as of 08:44, 7 October 2019
Expressible Wheat antimicrobial peptide 1b
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 261
Design Notes
The sequence of WAMP1b is preceded by a 6x His-Tag to facilitate its purification by immobilized metal affinity chromatography.
Source
The composite part was designed using the following parts previously added to the Registry: BBa_R0010, BBa_B0034, and BBa_B0015. The amino acid sequence for WAMP1b was retrieved from Odintsova et al., 2009.
References
1. Odintsova, T. I., Vassilevski, A. A., Slavokhotova, A. A., Musolyamov, A. K., Finkina, E. I., Khadeeva, N. V., … Egorov, T. A. (2009). A novel antifungal hevein-type peptide fromTriticum kiharaeseeds with a unique 10-cysteine motif. FEBS Journal, 276(15), 4266–4275. doi: 10.1111/j.1742-4658.2009.07135.x