Difference between revisions of "Part:BBa K3137001:Design"

 
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===References===
 
===References===
 +
Yasui R , Washizaki A , Furihata Y , et al. AbpA and AbpB provide anti-phage activity in Escherichia coli[J]. Genes & Genetic Systems, 2014, 89(2):51-60.

Revision as of 07:17, 7 October 2019


Improved RBS 4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequences of abpA and abpB overlap by 4 bp, which may facilitate the coupling of their expression at the translational level.


Source

abpA and abpB are obtained by PCR from the genome of E.coli BL21.

References

Yasui R , Washizaki A , Furihata Y , et al. AbpA and AbpB provide anti-phage activity in Escherichia coli[J]. Genes & Genetic Systems, 2014, 89(2):51-60.