Difference between revisions of "Part:BBa K2986005"
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===Design Notes=== | ===Design Notes=== | ||
How to use this polyprotein system is compatible with the need to target proteins-hGluc. To intuitively represent the expression of cytokines. | How to use this polyprotein system is compatible with the need to target proteins-hGluc. To intuitively represent the expression of cytokines. | ||
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===References=== | ===References=== | ||
Amrani, A. E. , Barakate, A. , Askari, B. M. , Li, X. , Roberts, A. G. , & Halpin, R. C. . (2004). Coordinate expression and independent subcellular targeting of multiple proteins from a single transgene. Plant Physiology, 135(1), 16-24. | Amrani, A. E. , Barakate, A. , Askari, B. M. , Li, X. , Roberts, A. G. , & Halpin, R. C. . (2004). Coordinate expression and independent subcellular targeting of multiple proteins from a single transgene. Plant Physiology, 135(1), 16-24. | ||
Revision as of 05:02, 7 October 2019
hGluc-P2A
A variety of conventional methods allow the expression of multiple foreign proteins in plants by transgene stacking or pyramiding. However, most of these approaches have significant drawbacks. We describe a novel alternative, using a single transgene to coordinate expression of multiple proteins that are encoded as a polyprotein capable of dissociating into component proteins on translation. We demonstrate that this polyprotein system is compatible with the need to target proteins to a variety of subcellular locations, either cotranslationally or posttranslationally. It can also be used to coordinate the expression of selectable marker genes and effect genes or to link genes that are difficult to assay to reporter genes that are easily monitored. The unique features of this polyprotein system are based on the novel activity of the 2A peptide of Foot-and-mouth disease virus (FMDV) that acts cotranslationally to effect a dissociation of the polyprotein while allowing translation to continue. This polyprotein system has many applications both as a research tool and for metabolic engineering and protein factory applications of plant biotechnology.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 67
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
How to use this polyprotein system is compatible with the need to target proteins-hGluc. To intuitively represent the expression of cytokines.
References
Amrani, A. E. , Barakate, A. , Askari, B. M. , Li, X. , Roberts, A. G. , & Halpin, R. C. . (2004). Coordinate expression and independent subcellular targeting of multiple proteins from a single transgene. Plant Physiology, 135(1), 16-24.