Difference between revisions of "Part:BBa K2986003"

Revision as of 03:01, 7 October 2019

light-switchable transactivator Basic part

Usage and Biology:

GVAPO is a synthetic light-switch transgene system, the transactivator binds promoters upon blue-light exposure and rapidly initiates transcription of target transgenes in mammalian cells and in mice. this transgene system provides a robust and convenient way to spatiotemporally control gene expression and can be used to manipulate many biological processes in living systems with minimal perturbation. Vivid (VVD), the smallest light-oxygen-voltage (LOV) domain–containing protein, forms a rapidly exchanging dimer upon blue-light activation. This part utilizes the DNA-binding property of a Gal4(65)-VVD fusion protein would be light-switchable, as light should induce dimerization of the fusion protein, enhance binding to the UASG sequence and activate transcription and removing the light should result in gradual dissociation of the dimers, DNA dissociation and inactivation.

[File:Light- dependent activation of Fluc reporters based on GAVP with different mutations to enhance dimerization.png ]]

Design Notes

How can we coupled this light-switch system with gene coded for the cytokines, which may stay in the cytosol or secrete to the outside of the cells. Whether changing another kind of cells can affect the effect of this system.

Source

This light-switch transgene system is form the lab of Xue Wang, Xianjun Chen & Yi Yang, they published Spatiotemporal control of gene expression by a light-switchable transgene system in Nature Methods 2012.

SequenceAndFeatures

References

Wang X , Chen X , Yang Y . Spatiotemporal control of gene expression by a light-switchable transgene system[J]. Nature Methods, 2012, 9(3):266-269.