Difference between revisions of "Part:BBa K2957000"

Revision as of 01:36, 7 October 2019

IL-8 (CXCL8)

Contents: IL-8 (CXCL-8), with Spec resistance

What is it?: A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)

What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 244
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2957000 short</partinfo>
-Contents: IL-8 (CXCL-8), with Spec resistance
+<b>Contents:</b> IL-8 (CXCL-8), with Spec resistance
-What is it? A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)
+<b>What is it?:</b> A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)
-What does it do? It codes for the chemokine, IL-8 (CXCL8), which is involved in inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.
+<b>What does it do?:</b> It codes for the chemokine, IL-8 (CXCL8), which is involved in inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.
-How to use it? You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
+<b>How to use it?:</b> You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
 <!-- Add more about the biology of this part here