Difference between revisions of "Part:BBa K2957002"

Revision as of 01:34, 7 October 2019

IL-8 (CXCL-8) with NeonGreen tag

Contents: IL-8 (CXCL-8), NeonGreen tag, with Carb resistance

What is it?: A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)

What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It has a NeonGreen tag attached.

How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. It also has a NeonGreen tag attached to it (which makes it different from pIG_047, BBa K779200) so this allows us to visualize it and determine transfection efficiency.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 460
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 460
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 234
Illegal BamHI site found at 476
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 460
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 460
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2957002 short</partinfo>
-Contents: IL-8 (CXCL-8), NeonGreen tag, with Carb resistance
+<b>Contents:</b> IL-8 (CXCL-8), NeonGreen tag, with Carb resistance
-What is it?: A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)
+<b>What is it?:</b> A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)
-What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It has a NeonGreen tag attached.
+<b>What does it do?:</b> It codes for the chemokine, IL-8 (CXCL8), which is involved in inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It has a NeonGreen tag attached.
-How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. It also has a NeonGreen tag attached to it (which makes it different from pIG_047, BBa K779200) so this allows us to visualize it and determine transfection efficiency.
+<b>How to use it?:</b> You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. It also has a NeonGreen tag attached to it (which makes it different from pIG_047, BBa K779200) so this allows us to visualize it and determine transfection efficiency.
 <!-- Add more about the biology of this part here