Difference between revisions of "Part:BBa K2996703:Design"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2996600 short</partinfo>
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<partinfo>BBa_K2996703 short</partinfo>
  
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<partinfo>BBa_K2996600 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2996703 SequenceAndFeatures</partinfo>
  
  
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===Source===
 
===Source===
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This sequence is amplified from E. coli.
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This sequence is amplified from E. coli K12 genome.
  
 
===References===
 
===References===
 
1.Bikard D , Jiang W , Samai P , et al. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system[J]. Nucleic Acids Research, 2013, 41(15):7429-7437.
 
1.Bikard D , Jiang W , Samai P , et al. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system[J]. Nucleic Acids Research, 2013, 41(15):7429-7437.

Revision as of 06:44, 6 October 2019


RpoA, DNA-directed RNA polymerase subunit alpha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 459
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

C-terminal is fused to dcas9 through a flexible linker.


Source

This sequence is amplified from E. coli K12 genome.

References

1.Bikard D , Jiang W , Samai P , et al. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system[J]. Nucleic Acids Research, 2013, 41(15):7429-7437.