Difference between revisions of "Part:BBa K3076500:Design"
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The genomic sequence of cusF gene was  extracted from NCBI (ACCESSION: NC_000913 REGION: 597131..597463)). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence. Double terminator was extracted from iGEM part registry (BBa_B0015) which is the most commonly used double terminator with high efficiency.
 
The genomic sequence of cusF gene was  extracted from NCBI (ACCESSION: NC_000913 REGION: 597131..597463)). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence. Double terminator was extracted from iGEM part registry (BBa_B0015) which is the most commonly used double terminator with high efficiency.
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===References===
 

Revision as of 15:18, 5 October 2019


dsDNA substrate with KanR gene for cusF knockout in E. coli by Lambda Red Recombineering


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A



References

The genomic sequence of cusF gene was extracted from NCBI (ACCESSION: NC_000913 REGION: 597131..597463)). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence. Double terminator was extracted from iGEM part registry (BBa_B0015) which is the most commonly used double terminator with high efficiency.