Difference between revisions of "Part:BBa K3111201"

 
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This part encodes mScarlet_DARPin929. The use of this part enabled us to study the binding and uptake of DARPin929 by conducting cell culture studies on SK-BR-3 HER2+ cells. The DARPin is flanked by red fluorescent protein mScarlet on the N-terminus which allowed visualisation of binding under confocal microscopy and a StrepII-tag downstream of this fusion, which allows for column-based purification of the expressed protein. It is expressed under a T7 promoter and a strong RBS.
  
 
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==Experimental Results==

Revision as of 15:08, 5 October 2019


mScarlet_DARPin929_StrepII

This part encodes mScarlet_DARPin929. The use of this part enabled us to study the binding and uptake of DARPin929 by conducting cell culture studies on SK-BR-3 HER2+ cells. The DARPin is flanked by red fluorescent protein mScarlet on the N-terminus which allowed visualisation of binding under confocal microscopy and a StrepII-tag downstream of this fusion, which allows for column-based purification of the expressed protein. It is expressed under a T7 promoter and a strong RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 791
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 652
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Results