Difference between revisions of "Part:BBa K3076200:Design"

 
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K3076250 short</partinfo>
+
<partinfo>BBa_K3076200 short</partinfo>
  
−
<partinfo>BBa_K3076250 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K3076200 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
 +
This part contains the coding sequence of vanabin2 gene. It was identified from literature review [1] and extracted from NCBI (ACCESSION: AB088204) starting from start codon to stop codon. Then the sequence was codon optimized for E. coli by ThermoFisher GeneArt platform.
 +
 +
===Design Consideration===
 
Overlapping sequence of pET28a multiple cloning site was added to flank the CDS for cloning by Hifi assembly.
 
Overlapping sequence of pET28a multiple cloning site was added to flank the CDS for cloning by Hifi assembly.
  
−
 
−
 
−
===Source===
 
−
 
−
This part contains the coding sequence of vanabin2 gene. It was identified from literature review [1] and extracted from NCBI (ACCESSION: AB088204) starting from start codon to stop codon. Then the sequence was codon optimized for E. coli by ThermoFisher GeneArt platform.
 
  
 
===References===
 
===References===
 +
[1] Ueki, T., Sakamoto, Y., Yamaguchi, N., & Michibata, H. (2003). Bioaccumulation of Copper Ions by Escherichia coli Expressing Vanabin Genes from the Vanadium-Rich Ascidian Ascidia sydneiensis samea. Applied and Environmental Microbiology, 69(11), 6442–6446. doi: 10.1128/aem.69.11.6442-6446.2003

Revision as of 14:50, 5 October 2019


Coding sequence of Vanabin2 gene from Ascidia sydneiensis samea


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 90
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains the coding sequence of vanabin2 gene. It was identified from literature review [1] and extracted from NCBI (ACCESSION: AB088204) starting from start codon to stop codon. Then the sequence was codon optimized for E. coli by ThermoFisher GeneArt platform.

Design Consideration

Overlapping sequence of pET28a multiple cloning site was added to flank the CDS for cloning by Hifi assembly.


References

[1] Ueki, T., Sakamoto, Y., Yamaguchi, N., & Michibata, H. (2003). Bioaccumulation of Copper Ions by Escherichia coli Expressing Vanabin Genes from the Vanadium-Rich Ascidian Ascidia sydneiensis samea. Applied and Environmental Microbiology, 69(11), 6442–6446. doi: 10.1128/aem.69.11.6442-6446.2003