Difference between revisions of "Part:BBa K3076100:Design"
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
The DNA sequence was modified to suit the synthesis by IDT while retaining the original amino acid sequence.
+
This part contains the genomic sequence of CgMT. It was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906) and then codon-optimized for E. coli by ThermoFisher GeneArt platform.
−
 
+
−
 
+
−
 
+
−
===Source===
+
−
 
+
−
NCBI
+
  
 
===References===
 
===References===
−
 
+
[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064
−
[1] NCBI gene ID 856450
+
−
 
+
−
[2] N Thirumoorthy, KT Manisenthil Kumar, A Shyam Sundar, L Panayappan, Malay Chatterjee (2007). Metallothionein: An overview. World journal of gastroenterology, ISSN 1007-9327
+
−
 
+
−
[3] Almaguer-Cantú V1, Morales-Ramos LH, Balderas-Rentería I. (2011) Biosorption of lead (II) and cadmium (II) using Escherichia coli genetically engineered with mice metallothionein I. Water Sci Technol. 2011;63(8):1607-13.
+
−
 
+
−
[4] Dziegiel, P., Pula, B., Kobierzycki, C., Stasiolek, M., Podhorska-Okolow, M. (2016), Metallothioneins in Normal and Cancer Cells, Springer International Publishing, DOI: 10.1007/978-3-319-27472-0
+
−
 
+
−
[5] Brenes-Pomales, A., Lindegren, G., & Lindegren, C. C. (1955). Gene Control of Copper-Sensitivity in Saccharomyces. Nature, 176(4487), 841-842. doi:10.1038/176841a0
+
−
 
+
−
[6] Adamo, G., Brocca, S., Passolunghi, S., Salvato, B., & Lotti, M. (2012). Laboratory evolution of copper tolerant yeast strains. Microbial Cell Factories, 11(1), 1. doi:10.1186/1475-2859-11-1
+
−
 
+
−
[7] Berka, T., Shatzman, A., Zimmerman, J., Strickler, J., & Rosenberg, M. (1988). Efficient expression of the yeast metallothionein gene in Escherichia coli. Journal of Bacteriology, 170(1), 21-26. doi:10.1128/jb.170.1.21-26.1988
+

Revision as of 14:34, 5 October 2019


Protein coding sequence for Saccharomyces cerevisiae metallothionein 1 (CUP1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains the genomic sequence of CgMT. It was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906) and then codon-optimized for E. coli by ThermoFisher GeneArt platform.

References

[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064