Difference between revisions of "Part:BBa K137068:Experience"
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===Applications of BBa_K137068=== | ===Applications of BBa_K137068=== | ||
====KatG+LAA is functional==== | ====KatG+LAA is functional==== | ||
| − | *Strain: SN0037 ( | + | *Construct: K137079 |
| + | *Strain: SN0037 (ΔkatE, katG) | ||
*Media: M9 + 2% glucose | *Media: M9 + 2% glucose | ||
*KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture. | *KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture. | ||
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{| border="1" | {| border="1" | ||
| − | !Hydrogen peroxide (mM)!! +AHL ( | + | !Hydrogen peroxide (mM)!! +AHL (100nM)!! -AHL |
|- | |- | ||
Revision as of 00:09, 5 September 2008
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Applications of BBa_K137068
KatG+LAA is functional
- Construct: K137079
- Strain: SN0037 (ΔkatE, katG)
- Media: M9 + 2% glucose
- KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture.
| Hydrogen peroxide (mM) | +AHL (100nM) | -AHL |
|---|---|---|
| 0.0 | Y | Y |
| 0.3 | Y | Y |
| 1.0 | Y | N |
| 3.0 | Y | N |
| 10.0 | N | N |
Y = Turbid growth after overnight incubation. N = No turbid growth after overnight incubation.
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