Difference between revisions of "Part:BBa K2996003:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Just be careful with the number of necleotides you are adding, since this device only works when the reading frame can be precisely regulated.
+
The structure of RSRL sequence is complicated and it is relatively short, we suggest direct synthesis when applying this biobrick.
 +
Also, be careful with the number of necleotides you are adding, since this device only works when the reading frame can be precisely regulated.
  
 +
===Source===
 +
RSRL cassette contains 69 bp of the leader2.1 and the adjacent CRISPR-spacer-CRISPR amplified from E. coli K12 str. MG.1655.
  
  
−
===Source===
 
  
−
The sequence of CRSIPR array is taken from that of E.coli K12. We simply synthesised this repeat-spacer-repeat-leader.
 
  
 
===References===
 
===References===
 +
1.Amlinger L , Hoekzema M , Wagner E G H , et al. Fluorescent CRISPR Adaptation Reporter for rapid quantification of spacer acquisition[J]. Scientific Reports, 2017, 7(1):10392.
 +
2.Díez-Villase?or, César, Guzmán, Noemí M, Almendros, Cristóbal, et al. CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli, [J]. RNA Biology, 2013, 10(5):792-802.

Latest revision as of 12:15, 4 October 2019


Tac promoter and operator plus RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The structure of RSRL sequence is complicated and it is relatively short, we suggest direct synthesis when applying this biobrick. Also, be careful with the number of necleotides you are adding, since this device only works when the reading frame can be precisely regulated.

Source

RSRL cassette contains 69 bp of the leader2.1 and the adjacent CRISPR-spacer-CRISPR amplified from E. coli K12 str. MG.1655.



References

1.Amlinger L , Hoekzema M , Wagner E G H , et al. Fluorescent CRISPR Adaptation Reporter for rapid quantification of spacer acquisition[J]. Scientific Reports, 2017, 7(1):10392. 2.Díez-Villase?or, César, Guzmán, Noemí M, Almendros, Cristóbal, et al. CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli, [J]. RNA Biology, 2013, 10(5):792-802.