Difference between revisions of "Part:BBa K2996001:Design"
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===Source=== | ===Source=== | ||
| − | + | The pRec plasmid containing cas1-cas2 is donated from Harris H. Wang from Columbia University. The cas1-cas2 cassette is amplified from NEB 10-beta. | |
| + | They are the only two Cas proteins found in all CRISPR–Cas systems, so cas1-cas2 are pretty conserved. | ||
===References=== | ===References=== | ||
Revision as of 10:22, 4 October 2019
cas1-cas2 complex
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 116
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 837
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
CAS
Source
The pRec plasmid containing cas1-cas2 is donated from Harris H. Wang from Columbia University. The cas1-cas2 cassette is amplified from NEB 10-beta. They are the only two Cas proteins found in all CRISPR–Cas systems, so cas1-cas2 are pretty conserved.