Difference between revisions of "Part:BBa K3098015"

Revision as of 10:11, 4 October 2019

sfGFP+Avi-tag+His-tag

iGEM19_WHU-China added Avi-tag to C-terminal of sfGFP to confirm the function of BirA enzyme(BBa_K2485001).sfGFP is codon optimized from BBa_K2804006.Avi-tag can perform normal function whether added to C-terminal or N-terminal of the peptide.Avi-tag can be specifically recognized by BirA enzyme in vitro/vivo to attach a biotin. Furthermore, this part can be used to produce biotinylated sfGFP for its purification and other signal detection/amplification possibilities in future versatile platform.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 25
Illegal AgeI site found at 148
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3098015 short</partinfo>
-iGEM19_WHU-China added Avi-tag to C-terminal of sfGFP to confirm the function of BirA enzyme(BBa_K2485001). Avi-tag can be specifically recognized by BirA enzyme in vitro/vivo to attach a biotin.
+iGEM19_WHU-China added Avi-tag to C-terminal of sfGFP to confirm the function of BirA enzyme(BBa_K2485001).sfGFP is codon optimized from BBa_K2804006.Avi-tag can perform normal function whether added to C-terminal or N-terminal of the peptide.Avi-tag can be specifically recognized by BirA enzyme in vitro/vivo to attach a biotin.
 Furthermore, this part can be used to produce biotinylated sfGFP for its purification and other signal detection/amplification possibilities in future versatile platform.