Difference between revisions of "Part:BBa K3221201"
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| − | + | MET3 promoter(pMET3) is a tight promoter which comes from Saccharomyces cerevisiae BY4742. The pMET3 regulates MET3 gene expression, which is a vital enzyme in the methionine biosynthetic pathway in Saccharomyces cerevisiae. This promoter can be regulated by methionine. The high concentration of methionine can inhibit the promoter’ s expression, and it can turn on when the methionine in the medium at a low concentration. Because of this characteristic, we used it in our delay expression system. | |
Revision as of 07:42, 4 October 2019
MET3 promoter(pMET3) is a tight promoter which comes from Saccharomyces cerevisiae BY4742. The pMET3 regulates MET3 gene expression, which is a vital enzyme in the methionine biosynthetic pathway in Saccharomyces cerevisiae. This promoter can be regulated by methionine. The high concentration of methionine can inhibit the promoter’ s expression, and it can turn on when the methionine in the medium at a low concentration. Because of this characteristic, we used it in our delay expression system.