Difference between revisions of "Part:BBa K137068:Experience"

(→‎Applications of BBa_K137068)
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**Media: M9 + 2% glucose
 
**Media: M9 + 2% glucose
 
**KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture.
 
**KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture.
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{| border="1"
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!Hydrogen peroxide (mM)!! +AHL (100mM)!! -AHL
 +
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|-
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|0.0|| Y || Y |
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 +
|-
 +
|0.3|| Y || Y |
 +
 +
|-
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|1.0|| Y || N |
 +
 +
|-
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|3.0|| Y || N |
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 +
|-
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|10.0|| N || N |
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 +
|}
  
 
===User Reviews===
 
===User Reviews===

Revision as of 19:39, 4 September 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K137068

  • KatG+LAA is functional
    • Strain: SN0037 (DE katE, katG)
    • Media: M9 + 2% glucose
    • KatG effectively protects E. coli from exogenous hydrogen peroxide. When expressed behind F2622 and induced with 100nM acylhomoserine lactone, the minimum inhibitory concentration (MIC) of SN0037 for hydrogen peroxide increases from 0.3 mM to 3mM. The MIC is defined as the concentration of hydrogen peroxide needed to prevent overnight turbid growth of a 1:50 dilution of a stationary phase culture.
Hydrogen peroxide (mM) +AHL (100mM) -AHL
0.0 Y
0.3 Y
1.0 Y
3.0 Y
10.0 N

User Reviews

UNIQc2c394f2a6d683e9-partinfo-00000000-QINU UNIQc2c394f2a6d683e9-partinfo-00000001-QINU