Difference between revisions of "Part:BBa K2996501:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We wanted a relatively strong terminator that can stop the transcription of DBD protein,thus lowering the leakage in the system,so we chose the terminator BBa_B0010 as the terminator.We designed new PCR primers to extend the original DNA sequence of DBD protein(BBa_K2996500),adding the DNA sequence of BBa_B0010 to the sequence.
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===Source===
 
===Source===

Revision as of 06:48, 3 October 2019


Terminator put downstream of zinc finger


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted a relatively strong terminator that can stop the transcription of DBD protein,thus lowering the leakage in the system,so we chose the terminator BBa_B0010 as the terminator.We designed new PCR primers to extend the original DNA sequence of DBD protein(BBa_K2996500),adding the DNA sequence of BBa_B0010 to the sequence.

Source

123

References