Difference between revisions of "Part:BBa K3078000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Sticky ends of BbsI and BsaI were designed as CTAG and CAAA. So any oligo wanted to be insert should start with CTAG and end with CAAA.
 
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===Source===
 
===Source===

Revision as of 03:47, 3 October 2019


HpaX


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 794
    Illegal BglII site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 412
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

BbsI site+BsaI site+RBS(BBa_J64022)+sfGFP(BBa_K2541400)+double terminator(BBa_B0015)

References