Difference between revisions of "Part:BBa K3034006"

 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3034006 short</partinfo>
 
<partinfo>BBa_K3034006 short</partinfo>
 +
 +
===Usage and Biology===
 +
 +
This part is a ciprofloxacin resistance cassette. It contains a strong promoter J23119(<html><a href='https://parts.igem.org/Part:BBa_J23119'>BBa_J23119</a></html>), RBS(<html><a href='https://parts.igem.org/Part:BBa_K1725309'>BBa_K1725309</a></html>), ciprofloxacin resistance gene ''qnrS1'' and a terminator(<html><a href='https://parts.igem.org/Part:BBa_B1006'>BBa_B1006</a></html>)(fig.1).
 +
 +
Ciprofloxacin resistance gene ''qnrS1'' is first found in Gram-negative strains. It's one of the plasmid-mediated quinolone resistance genes and it encodes pentapeptide duplicates of about 200 amino acids. These proteins can produce quinolone resistance through two different mechanisms of action. One is decreasing the number of quinolone target sites by reducing DNA binding to helicase or topoisomerase IV. The other one is to inhibit the binding of quinolone to enzymes by binding these proteins to helicase or topoisomerase.
 +
 +
[[File:T-UESTC-China-Framework.png|500px|thumb|center|'''fig.1''' The Framework of <html><a href='https://parts.igem.org/Part:BBa_K3034006'>BBa_K3034006</a></html>.]]
  
−
Ciprofloxacin resistance cassette
 
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3034006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3034006 SequenceAndFeatures</partinfo>

Revision as of 03:32, 2 October 2019


Ciprofloxacin resistance cassette

Usage and Biology

This part is a ciprofloxacin resistance cassette. It contains a strong promoter J23119(BBa_J23119), RBS(BBa_K1725309), ciprofloxacin resistance gene qnrS1 and a terminator(BBa_B1006)(fig.1).

Ciprofloxacin resistance gene qnrS1 is first found in Gram-negative strains. It's one of the plasmid-mediated quinolone resistance genes and it encodes pentapeptide duplicates of about 200 amino acids. These proteins can produce quinolone resistance through two different mechanisms of action. One is decreasing the number of quinolone target sites by reducing DNA binding to helicase or topoisomerase IV. The other one is to inhibit the binding of quinolone to enzymes by binding these proteins to helicase or topoisomerase.

fig.1 The Framework of BBa_K3034006.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]