Difference between revisions of "Part:BBa K2927010"

Revision as of 16:05, 1 October 2019

DNA template of crRNA 1 for LbCas12a

crRNA is crucial for the CRISPR Cas system. It is the short sequence that combines with Cas12a protein and forms a functional complex. Once crRNA binds with Cas12a protein, the complex capable to target and cleave targeted double-stranded DNA sequence (cis cleavage activity) and the cleavage of double-stranded DNA activates the cleavage of non-specific ssDNA (trans cleavage activity). In our project, we use crRNA for detecting a part sequence of vp72 membrane protein of African swine fever virus (ASFV).

When crRNA 1 binds with Cas12a protein, the protein may carry out cis-trans cleavage activity once it recognizes its target. By the way, though crRNA 1 is derived from ASFV, there is no possibility for virulence. The sequence of crRNA 1 is derived from p72 which is the capsid of ASFV. Therefore, the sequence of crRNA 1 is too short to be a functional protein. Thus, there is probably no risk for the spread out of ASFV from our project. Therefore, the design of this gene expression was reported in the check-in form and got approved.  

Sequence and Features