Difference between revisions of "Part:BBa K2992041"

Revision as of 10:40, 1 October 2019

5-UTR containing RBS for ha33 gene from C. botulinum

5-UTR containing RBS for ha33 gene from C. botulinum.

Usage and Biology

This parts entry represents the 5’-UTR containing the RBS for the hemagglutinin component of the botulinum neurotoxin complex from C. botulinum. Since ha33 is natural constituent of the BotR regulon, we chose this promoter to control the expression of our FAST reporter construct thus linking reporter fluorescence with botulinum neurotoxin production. In doing so, we hoped to generate our surrogate host strain as a model for predicting neurotoxin production in foodstuffs following food manufacturing processes.

Characterisation

Data incoming.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

Dupuy and Matamouros, 2006 Raffestin et al 2009 update

@@ Line 5: / Line 5: @@
 -UTR containing RBS for ha33 gene from <i>C. botulinum</i>.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This parts entry represents the 5’-UTR containing the RBS for the hemagglutinin component of the botulinum neurotoxin complex from <i>C. botulinum</i>. Since <i>ha33</i> is natural constituent of the BotR regulon, we chose this promoter to control the expression of our FAST reporter construct thus linking reporter fluorescence with botulinum neurotoxin production. In doing so, we hoped to generate our surrogate host strain as a model for predicting neurotoxin production in foodstuffs following food manufacturing processes.   <br><br>
+===Characterisation===
+Data incoming.
 <!-- -->
@@ Line 12: / Line 15: @@
 <partinfo>BBa_K2992041 SequenceAndFeatures</partinfo>
+===References===
+Dupuy  and Matamouros, 2006
+Raffestin et al 2009 update
 <!-- Uncomment this to enable Functional Parameter display