Difference between revisions of "Part:BBa K2918014"
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<partinfo>BBa_K2918014 short</partinfo> | <partinfo>BBa_K2918014 short</partinfo> | ||
Ribosomal Binding Site functional in both gram positives and gram negatives | Ribosomal Binding Site functional in both gram positives and gram negatives | ||
| − | + | Add more about the biology of this part here | |
===Usage and Biology=== | ===Usage and Biology=== | ||
This ribosomal binding site has been made by the use of the RBS calculator 2.0 by Sen Yang et al. This RBS functions both in gram negative (E. coli) and gram positive (B. subtilis). | This ribosomal binding site has been made by the use of the RBS calculator 2.0 by Sen Yang et al. This RBS functions both in gram negative (E. coli) and gram positive (B. subtilis). | ||
| − | + | <br> | |
| − | + | This part has been designed with Modular Cloning compatible overhangs (TACT - AATG). It makes use of the TypeIIs BsaI enzyme. | |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2918014 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2918014 SequenceAndFeatures</partinfo> | ||
Revision as of 19:26, 30 September 2019
Universal RBS
Ribosomal Binding Site functional in both gram positives and gram negatives
Add more about the biology of this part here
Usage and Biology
This ribosomal binding site has been made by the use of the RBS calculator 2.0 by Sen Yang et al. This RBS functions both in gram negative (E. coli) and gram positive (B. subtilis).
This part has been designed with Modular Cloning compatible overhangs (TACT - AATG). It makes use of the TypeIIs BsaI enzyme.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]