Difference between revisions of "Part:BBa S05497"

 
 
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<partinfo>BBa_S05497 short</partinfo>
 
<partinfo>BBa_S05497 short</partinfo>
  
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Construction intermediate
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Construction intermediate:
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This biobrick is the result of the binding of a constitutive promoter (BBa_J23101) and an RBS (BBa_B0034) by 3AAssembly,
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once linked to a CDS followed by a terminator, it will allow the normal expression of the gene
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iGEM Grenoble alpes has designated this biobrick to bind it to the biobrick (BBa_K3128032) OmpX mutated at the level of tyrosine, itself linked to the terminator in order to be able to express a clickable OmpX protein
  
 
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Latest revision as of 17:19, 29 September 2019


J23101:B0034

Construction intermediate: This biobrick is the result of the binding of a constitutive promoter (BBa_J23101) and an RBS (BBa_B0034) by 3AAssembly, once linked to a CDS followed by a terminator, it will allow the normal expression of the gene iGEM Grenoble alpes has designated this biobrick to bind it to the biobrick (BBa_K3128032) OmpX mutated at the level of tyrosine, itself linked to the terminator in order to be able to express a clickable OmpX protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]