Difference between revisions of "Part:BBa K3064022"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | HA-tag, derived from the HA-molecule corresponding to amino acids 98-106, is used as a | + | HA-tag, derived from the HA-molecule corresponding to amino acids 98-106, is used in expression vectors as a epitope tag. It can be used in the detection, isolation and purification of target proteins.<Br/> |
Trim 21 belongs the family of TRIM and exist in many eukaryotic cells. MmuTrim 21 comes from mouse eukaryotic cells. It can bind IgG, IgM, IgA as a cytosolic Fc receptor. In intracellular cell, Trim 21 can orchestrates humoral immunity in the cytosolic environment and mediates neutralization by degrading antibody-coated viruses in the proteasome as a E3 ubiquitin-protein ligase.<Br/> | Trim 21 belongs the family of TRIM and exist in many eukaryotic cells. MmuTrim 21 comes from mouse eukaryotic cells. It can bind IgG, IgM, IgA as a cytosolic Fc receptor. In intracellular cell, Trim 21 can orchestrates humoral immunity in the cytosolic environment and mediates neutralization by degrading antibody-coated viruses in the proteasome as a E3 ubiquitin-protein ligase.<Br/> | ||
Revision as of 03:06, 29 September 2019
HA-tag->mmuTrim21
This part can be used to test some certain expressed proteins that can bind with mmuTrim21.
Usage and Biology
HA-tag, derived from the HA-molecule corresponding to amino acids 98-106, is used in expression vectors as a epitope tag. It can be used in the detection, isolation and purification of target proteins.
Trim 21 belongs the family of TRIM and exist in many eukaryotic cells. MmuTrim 21 comes from mouse eukaryotic cells. It can bind IgG, IgM, IgA as a cytosolic Fc receptor. In intracellular cell, Trim 21 can orchestrates humoral immunity in the cytosolic environment and mediates neutralization by degrading antibody-coated viruses in the proteasome as a E3 ubiquitin-protein ligase.
This composite parts can be used to test the degradation of some certain targeting proteins, antibody-antigen complex and receptor-ligand complex with more convenience.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 110
Illegal BamHI site found at 813 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 211
- 1000COMPATIBLE WITH RFC[1000]
References
1.Stian Foss, Ruth Watkinson, Inger Sandlie, et al. TRIM21: a cytosolic Fc receptor with broad antibody isotype specificity[J]. Immunological Reviews, 2015, 268(1):328-339.
2.William A. McEwan, Leo C. James. Progress in Molecular Biology and Translational Science, 2015, 129:167-187.
3.HA-tag[G/OL]. https://en.wikipedia.org/wiki/HA-tag , 2019.