Difference between revisions of "Part:BBa K2653003"
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| − | The bicistronic P2A. | + | The bicistronic P2A peptide is a kind of self-cleaved sequence, which breaks when translated. The protein expressed by the same mRNA is separated before and after to produce two proteins, which can be used to construct the polycis vector and realize the co-translation of two proteins under the control of the same promoter |
Latest revision as of 02:36, 29 September 2019
P2A
The bicistronic P2A peptide is a kind of self-cleaved sequence, which breaks when translated. The protein expressed by the same mRNA is separated before and after to produce two proteins, which can be used to construct the polycis vector and realize the co-translation of two proteins under the control of the same promoter
Usage and Biology
Self-cleavage Bicistronic or multicistronic are widely used in expression of more than one gene at the same time. These molecules shapes only a small size, but has amazingly high cleavage efficiency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1]Jin H K, Lee S R, Li L H, et al. High Cleavage Efficiency of a 2A Peptide Derived from Porcine, Teschovirus-1 in Human Cell Lines, Zebrafish and Mice[J]. Plos One, 2011, 6(4):e18556.